Antisense Technology

Potent Inhibition of microRNA in vivo without Degradation.

Davis S, Propp S, Freier S, Jones L, Serra M, Kinberger G, Bhat B, Swayze E, Bennett F, Esau C.

Nucleic Acids Res. 2009 Jan;37(1):70-7.

Human Dicer Binds Short Single-strand and Double-strand RNA with High Affinity and Interacts with Different Regions of the Nucleic Acids.

Lima WF, Murray H, Nichols JG, Wu H, Sun H, Prakash TP, Berdeja AR, Gaus HJ, Crooke ST.

J Biol Chem. 2008 November [Epub ahead of print].

3' End Processing of a Long Nuclear-retained Noncoding RNA Yields a tRNA-like Cytoplasmic RNA.

Wilusz JE, Freier SM, Spector DL.

Cell. 2008 November;135(5):919-932.

MicroRNA 21 Promotes Glioma Invasion by Targeting Matrix Metalloproteinase Regulators.

Gabriely G, Wurdinger T, Kesari S, Esau CC, Burchard J, Linsley PS, Krichevsky AM.

Mol Cell Biol. 2008 Sep;28(17):5369-80.

Antisense Transcripts Are Targets for Activating Small RNAs

Schwartz JC, Younger ST, Nguyen N, Hardy DB, Monia BP, Corey DR, Janowski BA.

Nature Structural & Molecular Biology . 2008 August;15(8):842-848.

Targeted Depletion of Hepatic ACAT2-driven Cholesterol Esterification Reveals a Non-biliary Route for Fecal Neutral Sterol Loss.

Brown JM, Bell TA 3rd, Alger HM, Sawyer JK, Smith TL, Kelley K, Shah R, Wilson MD, Davis MA, Lee RG, Graham MJ, Crooke RM, Rudel LL.

J Biol Chem. 2008 Apr;283(16):10522-34.

Interferon-gamma Induces X-linked Inhibitor of Apoptosis-associated Factor-1 and Noxa Expresion and Potentiates Human Vascular Smooth Muscle Cell Apoptosis by STAT3 Activation.

Bai Y, Ahmad U, Wang Y, Li JH, Choy JC, Kim RW, Kirkiles-Smith N, Maher SE, Karras JG, Bennett CF, Bothwell AL, Pober JS, Tellides G.

J Biol Chem. 2008 Mar 14;283(11):6832-42.

Inhibition of microRNA with antisense oligonucleotides.

Esau CC.

PMID. 2008 Jan;44(1):55-60.

Regulation and Targeting of Eg5, a Mitotic Motor Protein in Blast Crisis CML: Overcoming Imatinib Resistance

Carter BZ, Mak DH, Shi Y, Schober WD, Wang RY, Konopleva M, Koller E, Dean NM, Andreeff M.

Cell Cycle. 2006 Oct;5(19):2223-9.

MicroRNA-143 Regulates Adipocyte differentiation.

Esau C, Kang X, Peralta E, Hanson E, Marcusson EG, Ravichandran LV, Sun Y, Koo S, Perera RJ, Jain R, Dean NM, Freier SM, Bennett CF, Lollo B, Griffey R.

J Biol Chem. 2004 Dec 10;279(50):52361-5.

PTEN Activation Contributes to Tumor Inhibition by Trastuzumab, and Loss of PTEN Predicts Trastuzumab Resistance in Patients.

Nagata Y, Lan KH, Zhou X, Tan M, Esteva FJ, Sahin AA, Klos KS, Li P, Monia BP, Nguyen NT, Hortobagyi GN, Hung MC, Yu D.

Cancer Cell. 2004 Aug;6(2):117-27.

Activation of SRC Tyrosine Kinases in Response to ICAM-1 Ligation in Pulmonary Microvascular Endothelial Cells.

Wang Q, Pfeiffer GR 2nd, Gaarde WA.

J Biol Chem. 2003 Nov 28;278(48):47731-43.

Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A comparative analysis.

Vickers, T.A., Koo, S., Bennett, C.F., Crooke, S.T., Dean, N.M., Baker, B.F.

J Biol. Chem. 278(9):7108-18, 2003.

Constitutive stat3 activity up-regulates VEGF expression and tumor angiogenesis.

Niu, G., K.L. Wright, M. Huang, L. Song, E. Haura, J. Turkson, S. Zhang, T. Wang, D. Sinibaldi, D. Coppola, R. Heller, L.M. Ellis, J. Karras, J. Broomberg, D. Pardoll, R. Jove, and H. Yu.

Oncogene. 2002 Mar 27;21(13):2000-8.

Systematically delivered antisense oligomers upregulate gene expression in mouse tissues.

Sazani, P., Gemignani, F., Kang, S-H, Maier, M.A., Manoharan, M. Persmark, M., Bortner, D., and Kole, R.

Nat. Biotechnol. 20: 1228-1233, 2002.

PTEN, but not SHIP and SHIP2, suppresses the PI3K/Akt pathway and induces growth inhibition and apoptosis of myeloma cells.

Choi, Y. Zhang, J., Murga, C. Yu, H., Koller, E., Monia, B.P., Gutkind, J.S., and Li, W

Oncogene. 21:(34)5289-300, 2002.

Holbrook, N.J. Targets of c-Jun NH(2)-terminal Kinase 2-mediated Tumor Growth Regulation Revealed by Serial Analysis of Gene Expression.

Potapova, O., Anisimov, S.V., Gorospe, M., Dougherty, R.H., Gaarde, W.A., Boheler, K.R.

Cancer Res. 62(11):3257-3263, 2002.

Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase.

Bulavin, D.V., Higashimoto, Y., Popoff, I.J., Gaarde, W.A., Basrur, V., Potapova, O. Appella, E., and Fornace, Jr., A.J.

Nature. 2001 May 3;411(6833):102-7.

Functional genomics and target validation approaches using antisense oligonucleotides technology

Dean, N.M.

Curr Opin Biotechnol. 12(6):622-5, 2001.

Pharmacokinetics of phosphorothioate antisense oligodeoxynucleotides.

Geary, R.S., Yu, R.Z., Levin, A.A.

Curr Opin Investig Drugs. 2(4):562-573, 2001.

Identification of an estrogen-inducible phosphatase (pp5) that converts mcf-7 human breast carcinoma cells into an estrogen-independent phenotype when expressed constitutively.

Urban, G., Golden, T., Aragon, I. V., Scammell, J. G., Dean, N. M., and Honkanen, R. E.

J Biol Chem. 276(29):27638-27646, 2001.

Deletion of individual exons and induction of soluble murine interleukin-5 receptor-alpha chain expression through antisense oligonucleotide-mediated redirection of pre-mrna splicing.

Karras, J. G., McKay, R. A., Dean, N. M., and Monia, B. P.

Mol Pharmacol. 58(2):380-387, 2000.

Progress in antisense technology: The end of the beginning.

Crooke, S.T.

Methods Enzymol. 313(Antisense Technology, Part A):3-45, 2000.

Molecular mechanisms of action of antisense drugs

Crooke, S.T.

Biochim Biophys Aca.1489 (1): 31-43, 1999.

Induction of endogenous Bcl-xS through the control of Bcl-x pre-mRNA splicing by antisense oligonucleotides.

Taylor, J. K., Zhang, Q. Q., Wyatt, R. and Dean, N. M

Nature Biotechnology.17(11):1097-1100, 1999.

Properties of cloned and expressed human RNase H.

Wu, H., Lima, W.F. and Crooke, S.T.

J. Biol. Chem. 274(40)28270-28278, 1999.

Pleiotropic cell-division defects and apoptosis induced by interference with survivin function.

Li, F., Ackermann, E.J., Bennett, C.F., Rothermel, A.L., Plescia, J., Tognin, S., Villa, A., Marchisio, P.C., and Altieri, D.C.

Nature Cell Biol. 1:461-466, 1999.

Antisense Therapeutics.

Crooke, S.T.

Biotechnol Genet Eng Rev. 1998;15:121-57.

2'-O-(2-methoxy)ethyl-modified anti-intercellular adhesion molecule 1 (ICAM-1) oligonucleotides selectively increase the ICAM-1 mRNA level and inhibit formation of the ICAM-1 translation initiation complex in human umbilical vein endothelial cells.

Baker, B. F., Lot, S. S., Condon, T. P., Cheng-Flournoy, S., Lesnik, E. A., Sasmor, H. M., and Bennett, C. F

Journal of Biological Chemistry. 272(18):11994-12000, 1997

PTP1B antisense-treated mice show regulation of genes involved in lipogenesis in liver and fat.

Waring, J.F., Ciurlionis, R., Clampit, J.E., Morgan, S., Gum, R.J., Jolly, R.A., Kroeger, P., Frost, L., Trevillyan, J., Zinker, B.A., Jirousek, M., Ulrich, R.G., Rondinone, C.M.

Molecular and Cellular Endocrinology. 203: 155-168, 2003.